deep vent high fidelity dna polymerase (New England Biolabs)
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Deep Vent High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/high+fidelity+deep+vent+dna+polymerase/Deep+Vent+DNA+Polymerase/pmc03552960-63-9-15
Average 96 stars, based on 3337 article reviews
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Amplification:Article Title: Trout CYP1A3 Gene: Recognition of Fish DNA Motifs by Mouse Regulatory Proteins. Article Snippet: Transcriptional up-regulation of mammalian CYP1A1 genes by dioxin is known to require binding of dioxin to the Ah receptor (AHR), subsequent interaction of this ligand-receptor complex with the AHR nuclear translocator (ARNT), and binding of this heterodimer to aromatic hydrocarbon response elements (AHREs) located in the 58 flanking sequences.. From the rainbow trout (Oncorhyncus mykiss), we have isolated and sequenced the CYP1A3 gene—spanning 4.0 kb and containing seven exons and six introns—and 1897 bp of the 58 flanking region.. The transcription start site was determined by primer extension analysis. Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Polymerase Chain Reaction:Article Title: Trout CYP1A3 Gene: Recognition of Fish DNA Motifs by Mouse Regulatory Proteins. Article Snippet: Transcriptional up-regulation of mammalian CYP1A1 genes by dioxin is known to require binding of dioxin to the Ah receptor (AHR), subsequent interaction of this ligand-receptor complex with the AHR nuclear translocator (ARNT), and binding of this heterodimer to aromatic hydrocarbon response elements (AHREs) located in the 58 flanking sequences.. From the rainbow trout (Oncorhyncus mykiss), we have isolated and sequenced the CYP1A3 gene—spanning 4.0 kb and containing seven exons and six introns—and 1897 bp of the 58 flanking region.. The transcription start site was determined by primer extension analysis. Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-tagged Collagen Constructs Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro- Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the luciferase gene. .. All PCR amplifications were done using Article Title: Amyotrophic lateral sclerosis-associated TDP-43 mutation Q331K prevents nuclear translocation of XRCC4-DNA ligase 4 complex and is linked to genome damage-mediated neuronal apoptosis Article Snippet: Expression plasmids The FLAG-TDP-43 WT pcDNA 3.1(+) expression plasmid was a kind gift from Prof. Paul Taylor (St. Jude Children’s Research Hospital; Memphis, TN). pCW-Cas9 was a gift from Eric Lander and David Sabatini (Addgene plasmid # 50661). .. The TDP-43 coding DNA sequence (CDS) along with the N-terminal 1X FLAG tag sequence was PCR-amplified from FLAG-TDP-43 pcDNA 3.1 vector using Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-Tagged Collagen Constructs. Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro-Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI, USA) to replace the luciferase gene. .. All PCR amplifications were performed using Article Title: Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri Article Snippet: PCR purification, gel extraction and plasmid purification kits were obtained from Qiagen. .. Construct:Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-tagged Collagen Constructs Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro- Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the luciferase gene. .. All PCR amplifications were done using Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-Tagged Collagen Constructs. Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro-Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI, USA) to replace the luciferase gene. .. All PCR amplifications were performed using DNA Sequencing:Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-tagged Collagen Constructs Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro- Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the luciferase gene. .. All PCR amplifications were done using Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-Tagged Collagen Constructs. Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro-Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI, USA) to replace the luciferase gene. .. All PCR amplifications were performed using Sequencing:Article Title: Amyotrophic lateral sclerosis-associated TDP-43 mutation Q331K prevents nuclear translocation of XRCC4-DNA ligase 4 complex and is linked to genome damage-mediated neuronal apoptosis Article Snippet: Expression plasmids The FLAG-TDP-43 WT pcDNA 3.1(+) expression plasmid was a kind gift from Prof. Paul Taylor (St. Jude Children’s Research Hospital; Memphis, TN). pCW-Cas9 was a gift from Eric Lander and David Sabatini (Addgene plasmid # 50661). .. The TDP-43 coding DNA sequence (CDS) along with the N-terminal 1X FLAG tag sequence was PCR-amplified from FLAG-TDP-43 pcDNA 3.1 vector using Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the FLAG-tag:Article Title: Amyotrophic lateral sclerosis-associated TDP-43 mutation Q331K prevents nuclear translocation of XRCC4-DNA ligase 4 complex and is linked to genome damage-mediated neuronal apoptosis Article Snippet: Expression plasmids The FLAG-TDP-43 WT pcDNA 3.1(+) expression plasmid was a kind gift from Prof. Paul Taylor (St. Jude Children’s Research Hospital; Memphis, TN). pCW-Cas9 was a gift from Eric Lander and David Sabatini (Addgene plasmid # 50661). .. The TDP-43 coding DNA sequence (CDS) along with the N-terminal 1X FLAG tag sequence was PCR-amplified from FLAG-TDP-43 pcDNA 3.1 vector using other:Article Title: Amyotrophic lateral sclerosis-associated TDP-43 mutation Q331K prevents nuclear translocation of XRCC4-DNA ligase 4 complex and is linked to genome damage-mediated neuronal apoptosis Article Snippet: Quantitation of DNA strand breaks by LA-PCR assay Genomic DNA was isolated from SH-SY5Y cells using the Qiagen Blood and Tissue kit (#69504, Qiagen, Germany), and DNA was quantified using a nanodrop spectrophotometer (DeNovix DS-11). shRNA:Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Mutagenesis:Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Clone Assay:Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Plasmid Preparation:Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Expressing:Article Title: Schlafen 11 Restricts Flavivirus Replication Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the Cloning:Article Title: Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri Article Snippet: PCR purification, gel extraction and plasmid purification kits were obtained from Qiagen. .. |
