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deep vent high fidelity dna polymerase  (New England Biolabs)


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    Structured Review

    New England Biolabs deep vent high fidelity dna polymerase
    Deep Vent High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 3337 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+fidelity+deep+vent+dna+polymerase/Deep+Vent+DNA+Polymerase/pmc03552960-63-9-15
    Average 96 stars, based on 3337 article reviews
    deep vent high fidelity dna polymerase - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: Trout CYP1A3 Gene: Recognition of Fish DNA Motifs by Mouse Regulatory Proteins.
    Article Snippet: Transcriptional up-regulation of mammalian CYP1A1 genes by dioxin is known to require binding of dioxin to the Ah receptor (AHR), subsequent interaction of this ligand-receptor complex with the AHR nuclear translocator (ARNT), and binding of this heterodimer to aromatic hydrocarbon response elements (AHREs) located in the 58 flanking sequences.. From the rainbow trout (Oncorhyncus mykiss), we have isolated and sequenced the CYP1A3 gene—spanning 4.0 kb and containing seven exons and six introns—and 1897 bp of the 58 flanking region.. The transcription start site was determined by primer extension analysis.

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. (iii) N- and C-terminal Slfn11 mutant expression plasmids. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Polymerase Chain Reaction:

    Article Title: Trout CYP1A3 Gene: Recognition of Fish DNA Motifs by Mouse Regulatory Proteins.
    Article Snippet: Transcriptional up-regulation of mammalian CYP1A1 genes by dioxin is known to require binding of dioxin to the Ah receptor (AHR), subsequent interaction of this ligand-receptor complex with the AHR nuclear translocator (ARNT), and binding of this heterodimer to aromatic hydrocarbon response elements (AHREs) located in the 58 flanking sequences.. From the rainbow trout (Oncorhyncus mykiss), we have isolated and sequenced the CYP1A3 gene—spanning 4.0 kb and containing seven exons and six introns—and 1897 bp of the 58 flanking region.. The transcription start site was determined by primer extension analysis.

    Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-tagged Collagen Constructs
    Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro- Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the luciferase gene. .. All PCR amplifications were done using high fidelity Deep Vent DNA polymerase (New England Biolabs) and sequences of constructs confirmed by DNA sequencing. .. Unless stated otherwise tissue culture reagents were obtained from Gibco (Life Technologies Inc., Grand Island, NY) or Mediatech Inc. (Herndon, VA).

    Article Title: Amyotrophic lateral sclerosis-associated TDP-43 mutation Q331K prevents nuclear translocation of XRCC4-DNA ligase 4 complex and is linked to genome damage-mediated neuronal apoptosis
    Article Snippet: Expression plasmids The FLAG-TDP-43 WT pcDNA 3.1(+) expression plasmid was a kind gift from Prof. Paul Taylor (St. Jude Children’s Research Hospital; Memphis, TN). pCW-Cas9 was a gift from Eric Lander and David Sabatini (Addgene plasmid # 50661). .. The TDP-43 coding DNA sequence (CDS) along with the N-terminal 1X FLAG tag sequence was PCR-amplified from FLAG-TDP-43 pcDNA 3.1 vector using high-fidelity Deep Vent DNA polymerase (# M0258, NEB, Ipswich, MA). ..

    Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-Tagged Collagen Constructs.
    Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro-Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI, USA) to replace the luciferase gene. .. All PCR amplifications were performed using high-fidelity Deep Vent DNA polymerase (New England Biolabs, Ipswich, MA, USA) and sequences of constructs confirmed by DNA sequencing. .. Cell culture and cell lines Unless stated otherwise, tissue culture reagents were obtained from Gibco (Life Technologies Inc., Grand Island, NY, USA) or Mediatech Inc. (Herndon, VA, USA).

    Article Title: Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri
    Article Snippet: PCR purification, gel extraction and plasmid purification kits were obtained from Qiagen. .. High-fidelity Deep Vent DNA Polymerase (New England Biolabs) was used to generate PCR products for cloning. ..

    Construct:

    Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-tagged Collagen Constructs
    Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro- Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the luciferase gene. .. All PCR amplifications were done using high fidelity Deep Vent DNA polymerase (New England Biolabs) and sequences of constructs confirmed by DNA sequencing. .. Unless stated otherwise tissue culture reagents were obtained from Gibco (Life Technologies Inc., Grand Island, NY) or Mediatech Inc. (Herndon, VA).

    Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-Tagged Collagen Constructs.
    Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro-Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI, USA) to replace the luciferase gene. .. All PCR amplifications were performed using high-fidelity Deep Vent DNA polymerase (New England Biolabs, Ipswich, MA, USA) and sequences of constructs confirmed by DNA sequencing. .. Cell culture and cell lines Unless stated otherwise, tissue culture reagents were obtained from Gibco (Life Technologies Inc., Grand Island, NY, USA) or Mediatech Inc. (Herndon, VA, USA).

    DNA Sequencing:

    Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-tagged Collagen Constructs
    Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro- Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI) to replace the luciferase gene. .. All PCR amplifications were done using high fidelity Deep Vent DNA polymerase (New England Biolabs) and sequences of constructs confirmed by DNA sequencing. .. Unless stated otherwise tissue culture reagents were obtained from Gibco (Life Technologies Inc., Grand Island, NY) or Mediatech Inc. (Herndon, VA).

    Article Title: Live Imaging of Type I Collagen Assembly Dynamics in Osteoblasts Stably Expressing GFP and mCherry-Tagged Collagen Constructs.
    Article Snippet: A puromycin selection vector (pGL3-puro) was made by subcloning the fragment containing promoter-puro cDNA from the pMSCV-puro-Col1a2 vector into the pGL3-basic vector (Promega Corporation, Madison, WI, USA) to replace the luciferase gene. .. All PCR amplifications were performed using high-fidelity Deep Vent DNA polymerase (New England Biolabs, Ipswich, MA, USA) and sequences of constructs confirmed by DNA sequencing. .. Cell culture and cell lines Unless stated otherwise, tissue culture reagents were obtained from Gibco (Life Technologies Inc., Grand Island, NY, USA) or Mediatech Inc. (Herndon, VA, USA).

    Sequencing:

    Article Title: Amyotrophic lateral sclerosis-associated TDP-43 mutation Q331K prevents nuclear translocation of XRCC4-DNA ligase 4 complex and is linked to genome damage-mediated neuronal apoptosis
    Article Snippet: Expression plasmids The FLAG-TDP-43 WT pcDNA 3.1(+) expression plasmid was a kind gift from Prof. Paul Taylor (St. Jude Children’s Research Hospital; Memphis, TN). pCW-Cas9 was a gift from Eric Lander and David Sabatini (Addgene plasmid # 50661). .. The TDP-43 coding DNA sequence (CDS) along with the N-terminal 1X FLAG tag sequence was PCR-amplified from FLAG-TDP-43 pcDNA 3.1 vector using high-fidelity Deep Vent DNA polymerase (# M0258, NEB, Ipswich, MA). ..

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. (iii) N- and C-terminal Slfn11 mutant expression plasmids. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    FLAG-tag:

    Article Title: Amyotrophic lateral sclerosis-associated TDP-43 mutation Q331K prevents nuclear translocation of XRCC4-DNA ligase 4 complex and is linked to genome damage-mediated neuronal apoptosis
    Article Snippet: Expression plasmids The FLAG-TDP-43 WT pcDNA 3.1(+) expression plasmid was a kind gift from Prof. Paul Taylor (St. Jude Children’s Research Hospital; Memphis, TN). pCW-Cas9 was a gift from Eric Lander and David Sabatini (Addgene plasmid # 50661). .. The TDP-43 coding DNA sequence (CDS) along with the N-terminal 1X FLAG tag sequence was PCR-amplified from FLAG-TDP-43 pcDNA 3.1 vector using high-fidelity Deep Vent DNA polymerase (# M0258, NEB, Ipswich, MA). ..

    other:

    Article Title: Amyotrophic lateral sclerosis-associated TDP-43 mutation Q331K prevents nuclear translocation of XRCC4-DNA ligase 4 complex and is linked to genome damage-mediated neuronal apoptosis
    Article Snippet: Quantitation of DNA strand breaks by LA-PCR assay Genomic DNA was isolated from SH-SY5Y cells using the Qiagen Blood and Tissue kit (#69504, Qiagen, Germany), and DNA was quantified using a nanodrop spectrophotometer (DeNovix DS-11).

    shRNA:

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. (iii) N- and C-terminal Slfn11 mutant expression plasmids. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Mutagenesis:

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. (iii) N- and C-terminal Slfn11 mutant expression plasmids. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Clone Assay:

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. (iii) N- and C-terminal Slfn11 mutant expression plasmids. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Plasmid Preparation:

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. (iii) N- and C-terminal Slfn11 mutant expression plasmids. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Expressing:

    Article Title: Schlafen 11 Restricts Flavivirus Replication
    Article Snippet: .. The newly created shRNA escape mutant cDNA was then amplified using the high-fidelity Deep Vent DNA polymerase (New England Biolabs), digested with SbfI and SpeI restriction enzymes, and cloned into unique SbfI-SpeI sites in the pTRIP-IRES-P HIV-1-derived transfer plasmid, and the sequence was verified. (iii) N- and C-terminal Slfn11 mutant expression plasmids. .. The pTRIP-IRES-P-Slfn11-shRNA-resistant plasmid was used as the template to generate the Slfn11 truncated mutants using the QuikChange Lightning site-directed mutagenesis kit (Agilent Technologies).

    Cloning:

    Article Title: Prediction of CsrA-regulating small RNAs in bacteria and their experimental verification in Vibrio fischeri
    Article Snippet: PCR purification, gel extraction and plasmid purification kits were obtained from Qiagen. .. High-fidelity Deep Vent DNA Polymerase (New England Biolabs) was used to generate PCR products for cloning. ..



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    A) Schematic illustration of the mouse Ins2 gene with promoter region (blue), exon 1 (yellow), intron 1 (white), and exon 2 (green) showing the positions of CpG sites and the primers used in this study. Black arrows represent the bisulfite-specific primers (BSP) that amplify both methylated and unmethylated <t>DNA.</t> Red arrows represent methylation-specific primers (MSP) that amplify unmethylated but not methylated DNA. B) Gel electrophoresis (3% agarose) of PCR <t>products</t> <t>amplified</t> by reactions using different primer sets and the cloned Ins2 gene as template. The clone was methylated (M) or sham methylated (N) and bisulfite-treated prior to use in the reactions. NTC means non-template control. TSS indicates the transcription starting site.
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    Image Search Results


    A) Schematic illustration of the mouse Ins2 gene with promoter region (blue), exon 1 (yellow), intron 1 (white), and exon 2 (green) showing the positions of CpG sites and the primers used in this study. Black arrows represent the bisulfite-specific primers (BSP) that amplify both methylated and unmethylated DNA. Red arrows represent methylation-specific primers (MSP) that amplify unmethylated but not methylated DNA. B) Gel electrophoresis (3% agarose) of PCR products amplified by reactions using different primer sets and the cloned Ins2 gene as template. The clone was methylated (M) or sham methylated (N) and bisulfite-treated prior to use in the reactions. NTC means non-template control. TSS indicates the transcription starting site.

    Journal: PLoS ONE

    Article Title: Development of a Quantitative Methylation-Specific Polymerase Chain Reaction Method for Monitoring Beta Cell Death in Type 1 Diabetes

    doi: 10.1371/journal.pone.0047942

    Figure Lengend Snippet: A) Schematic illustration of the mouse Ins2 gene with promoter region (blue), exon 1 (yellow), intron 1 (white), and exon 2 (green) showing the positions of CpG sites and the primers used in this study. Black arrows represent the bisulfite-specific primers (BSP) that amplify both methylated and unmethylated DNA. Red arrows represent methylation-specific primers (MSP) that amplify unmethylated but not methylated DNA. B) Gel electrophoresis (3% agarose) of PCR products amplified by reactions using different primer sets and the cloned Ins2 gene as template. The clone was methylated (M) or sham methylated (N) and bisulfite-treated prior to use in the reactions. NTC means non-template control. TSS indicates the transcription starting site.

    Article Snippet: The 842 bp fragment of the mouse insulin gene ( Ins2 ) from −480 to +362, containing nine CpG sites at positions −414, −182, and −171 in the promoter, +14 in exon 1, +121 in intron 1, and at +190, +310, +337, and +340 in exon 2, was PCR amplified from mouse gDNA using primers Ins2-pro-For and Ins2-pro-Rev ( ) and high-fidelity thermophilic DNA polymerase (Deep Vent DNA polymerase, NEB).

    Techniques: Methylation, Nucleic Acid Electrophoresis, Amplification, Clone Assay

    The unmethylated Ins2 gene plasmid was diluted in the presence or absence of 500 ng non-specific gDNA, bisulfite-treated, and used as template for PCR. A) 160, 80, 40, 20, 10, 5, and 2 copies of plasmid without non-specific DNA analyzed by qMSP using primer set P4/P6. B) Serial dilutions ranged from 10 8 to 10 copies of plasmid in the presence of non-specific gDNA analyzed by qMSP using primer set P4/P6. C) Serial dilution from 10 8 to 3 copies of plasmid in the absence of non-specific gDNA analyzed by qMSP using primer set P12/P13. D) Range of serial dilutions from 10 7 to 10 copies plasmid in the presence of non-specific gDNA analyzed by qMSP using primer set P12/P13. Mouse liver gDNA was used as non-specific DNA and NTC is the non-template control.

    Journal: PLoS ONE

    Article Title: Development of a Quantitative Methylation-Specific Polymerase Chain Reaction Method for Monitoring Beta Cell Death in Type 1 Diabetes

    doi: 10.1371/journal.pone.0047942

    Figure Lengend Snippet: The unmethylated Ins2 gene plasmid was diluted in the presence or absence of 500 ng non-specific gDNA, bisulfite-treated, and used as template for PCR. A) 160, 80, 40, 20, 10, 5, and 2 copies of plasmid without non-specific DNA analyzed by qMSP using primer set P4/P6. B) Serial dilutions ranged from 10 8 to 10 copies of plasmid in the presence of non-specific gDNA analyzed by qMSP using primer set P4/P6. C) Serial dilution from 10 8 to 3 copies of plasmid in the absence of non-specific gDNA analyzed by qMSP using primer set P12/P13. D) Range of serial dilutions from 10 7 to 10 copies plasmid in the presence of non-specific gDNA analyzed by qMSP using primer set P12/P13. Mouse liver gDNA was used as non-specific DNA and NTC is the non-template control.

    Article Snippet: The 842 bp fragment of the mouse insulin gene ( Ins2 ) from −480 to +362, containing nine CpG sites at positions −414, −182, and −171 in the promoter, +14 in exon 1, +121 in intron 1, and at +190, +310, +337, and +340 in exon 2, was PCR amplified from mouse gDNA using primers Ins2-pro-For and Ins2-pro-Rev ( ) and high-fidelity thermophilic DNA polymerase (Deep Vent DNA polymerase, NEB).

    Techniques: Plasmid Preparation, Serial Dilution